In order to guide the induced flow operation with negative pressure of highly deviated wells in offshore oilfields, provide a reasonable negative pressure design method, improve the effect of perforation channel cleaning, and ensure the productivity of oil and gas wells, combined with the characteristics of highly deviated well, the induced flow test with negative pressure of highly deviated well is carried out to analyze the impact of the induced flow timing on the channel cleaning, the results of four common negative pressure design methods are compared, the rationality of the design results of different negative pressure design methods is verified through the test results, and the minimal induced flow negative pressure design method is optimized. The test and analysis results show that: the permeability of the sandstone target after induced flow with negative pressure is 13% higher than the original permeability, and induced flow with negative pressure can effectively clean the perforation channel and remove perforation pollution; the permeability increases of the sandstone targets with induced flow immediately after perforation and induced flow after 24 h interval are 112% and 113% respectively, and the permeability increases under the two working conditions are basically the same; through comparison, when the target permeability of the sandstone is 5-34 mD (1 mD≈0.987×10?3 μm2), the minimal negative pressure designed by Berhmann is relatively large, and the minimal negative pressure designed by Core Lab, Conoc and VANN is relatively small. The research shows that the negative pressure backflow after an interval of 24 h will not reduce the cleaning effect of the channel, a single negative pressure backflow operation or dynamic negative pressure perforation and other technologies are required to clean the channel after the perforation of highly deviated wells. Based on the negative pressure backflow test results, the minimal negative pressure design model under the condition of low permeability reservoir is fitted, which can effectively guide the negative pressure backflow operation of the perforation of highly deviated wells, and is of great significance to improve the production of oil and gas wells.